Article
Synthesis of a wild-type and three mutant Cucurbita maxima trypsin inhibitor-encoding genes by a single-strand approach.
Gene - 15 Sept 1991
Botes D P, Qobose M D, Corfield V A
Abstract excerpt
A single-strand approach to gene assembly, based on a modification of an in vitro complementary oligodeoxyribonucleotide template-directed ligation of the desired sequence to a linearized vector [Chen et al., Nucleic Acids Res. 18 (1990) 871-878], is described. The gene coding for the wild-type Cucurbita maxima trypsin inhibitor of 29 amino acid residues [Bode et al., FEBS Lett. 242 (1989) 285-292], as well as...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Single-Stranded
- Molecular Sequence Data
- Mutation
- Plants
- Plasmids
- Trypsin Inhibitors