Article
Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system.
Journal of bioscience and bioengineering - 1 Apr 2006
Kataoka Yohei, Ozeki Shingo, Miyake Katsuhide, Iijima Shinji
Abstract excerpt
The cpsIaJ gene of Streptococcus agalactiae type Ia codes for beta-1,4-galactosyltransferase. In this study, the functional expression of His-tagged CpsIaJ in a baculovirus expression system was performed, because the efficient functional expression of this enzyme in Escherichia coli had been unsuccessful. Using a partially purified enzyme preparation, we found that the enzyme had a restricted substrate...
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