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Article

Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands.

Nucleic acids research - 11 Nov 1991

Ciccarelli R B, Gunyuzlu P, Huang J, Scott C, Oakes F T

Abstract excerpt

A new method is described for the direct construction of synthetic genes by applying a modified version of the polymerase chain reaction (PCR) to crude oligonucleotide mixtures made by automated solid phase DNA synthesis. Construction of the HIV-1 393 bp rev gene and the 655 bp nef gene by this method is illustrated. The sequences for the entire top and bottom strands of rev were each programmed into an automated...

Topics

Base Sequence
Cloning, Molecular
DNA
Electrophoresis, Agar Gel
Escherichia coli
Gene Expression
Genes, Synthetic
Genes, nef
Genes, rev
HIV-1
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Oligodeoxyribonucleotides

Plasmids

Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 1945885
DOI: 10.1093/nar/19.21.6007
PMCID: PMC329059

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1945885-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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