Article
Insertional gene synthesis, a novel method of assembling consecutive DNA sequences within specific sites in plasmids. Construction of the HIV-1 tat gene.
Nucleic acids research - 11 Mar 1990
Ciccarelli R B, Loomis L A, McCoon P E, Holzschu D L
Abstract excerpt
The construction of the HIV-1 tat gene using a novel method termed insertional gene synthesis (IGS) is described. IGS is used to assemble a gene or any DNA sequence in a stepwise manner within a plasmid containing a single stranded DNA phage origin of replication. The IGS method is based upon con...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Viral
- Escherichia coli
- Gene Expression Regulation, Viral
- Gene Products, tat
- Genes, Synthetic
- Genes, Viral
- Genes, tat
- HIV-1
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Plasmids
- Promoter Regions, Genetic