Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity.

Abstract excerpt

The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the utility of this method by selecting novel loss-of-function alleles of tatA from a pool of random...

Topics

• Escherichia coli
• Escherichia coli Proteins
• Membrane Transport Proteins
• Mutation