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Article

Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.

Journal of virology - 1 Nov 1992

Ray S, Anderson M E, Loeber G, McVey D, Tegtmeyer P

Abstract excerpt

We investigated the molecular properties of eight temperature-sensitive mutants of simian virus 40 large T antigen (tsA mutants). The mutants have single amino acid substitutions that block DNA replication at 39 to 41 degrees C in vivo. In vitro, five of the mutant proteins were highly sensitive to a brief heat shock at 39 degrees C, while the three remaining proteins were only partially sensitive at 41 degrees...

Topics

Adenosine Triphosphatases
Antigens, Viral, Tumor
Base Sequence
Cell-Free System
DNA Helicases
DNA Mutational Analysis
DNA Replication
DNA, Viral
Genes, Viral
Hot Temperature
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Protein Conformation

Simian virus 40

Source

Open in PubMed Open full text

Identifiers

PMID: 1328677
DOI: 10.1128/JVI.66.11.6509-6516.1992
PMCID: PMC240144

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1328677-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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