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Article

Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.

Journal of virology - 1 Nov 1992

Ray S, Anderson M E, Loeber G, McVey D, Tegtmeyer P

Abstract excerpt

We investigated the molecular properties of eight temperature-sensitive mutants of simian virus 40 large T antigen (tsA mutants). The mutants have single amino acid substitutions that block DNA replication at 39 to 41 degrees C in vivo. In vitro, five of the mutant proteins were highly sensitive to a brief heat shock at 39 degrees C, while the three remaining proteins were only partially sensitive at 41 degrees...

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Identifiers

PMID: 1328677
DOI: 10.1128/JVI.66.11.6509-6516.1992
PMCID: PMC240144

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