Article
Model for T-antigen-dependent melting of the simian virus 40 core origin based on studies of the interaction of the beta-hairpin with DNA.
Journal of virology - 1 May 2007
Kumar Anuradha, Meinke Gretchen, Reese Danielle K, Moine Stephanie, Phelan Paul J, Fradet-Turcotte Amélie, Archambault Jacques, Bohm Andrew, Bullock Peter A
Abstract excerpt
The interaction of simian virus 40 (SV40) T antigen (T-ag) with the viral origin has served as a model for studies of site-specific recognition of a eukaryotic replication origin and the mechanism of DNA unwinding. These studies have revealed that a motif termed the "beta-hairpin" is necessary for assembly of T-ag on the SV40 origin. Herein it is demonstrated that residues at the tip of the "beta-hairpin" are...
Topics
- Amino Acid Motifs
- Antigens, Viral, Tumor
- DNA Helicases
- DNA Replication
- DNA, Single-Stranded
- Models, Molecular
- Mutation
- Oligonucleotides
- Replication Origin
- Simian virus 40
- Virus Replication