Article
Construction of SV40 deletion mutants and delimitation of the binding domain for heat shock protein to the amino terminus of large T-antigen.
Virus research - 1 Mar 1994
Sawai E T, Rasmussen G, Butel J S
Abstract excerpt
SV40 large T-antigen (T-ag) mutants were generated using a cassette mutagenesis strategy and naturally occurring restriction sites. T-ag mutant constructs included internal in-frame deletions, frame-shift deletions that resulted in amino-terminal fragments, and internal initiation mutants that pr...
Topics
- 3T3 Cells
- Animals
- Antigens, Polyomavirus Transforming
- Cell Line
- Gene Deletion
- Gene Expression Regulation, Viral
- Heat-Shock Proteins
- Mice
- Mice, Inbred BALB C
- Mutation
- Protein Binding