Article
Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
Journal of virology - 1 Feb 1990
Parsons R, Anderson M E, Tegtmeyer P
Abstract excerpt
The simian virus 40 (SV40) core origin of replication consists of three functional domains. The sequence 5'-CACTACTTCTGGAATAG-3' with an imperfect inverted repeat (underlined), a palindrome with four 5'-GAGGC-3' pentanucleotide repeats, and a 17-base-pair A + T-rich segment. We have been able to assign primary functions to each domain. Remarkably, SV40 large T antigen melted the inverted repeat domain in the...
Topics
- Antigens, Polyomavirus Transforming
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA, Viral
- Deoxyribonuclease I
- Genes, Viral
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleotide Mapping