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Article

Thermally inactivated simian virus 40 tsA58 mutant T antigen cannot initiate viral DNA replication in vitro.

Journal of virology - 1 Dec 1990

Reynisdóttir I, O'Reilly D R, Miller L K, Prives C

Abstract excerpt

The mutation in the temperature-sensitive tsA58 mutant T antigen (Ala-438----Val) lies within the presumptive ATP-binding fold. We have constructed a recombinant baculovirus that expresses large quantities of the tsA58 T antigen in infected insect cells. The mutant T antigen mediated simian virus 40 origin-containing DNA (ori-DNA) synthesis in vitro to nearly the same extent as similar quantities of wild-type T...

Topics

Animals
Antigens, Polyomavirus Transforming
Cell Line
DNA Replication
DNA, Viral
DNA-Binding Proteins
Deoxyribonuclease I
HeLa Cells
Hot Temperature
Humans
Insecta

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Kinetics

Mutation

Plasmids

Simian virus 40

Temperature

Source

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Identifiers

PMID: 2173789
DOI: 10.1128/JVI.64.12.6234-6245.1990
PMCID: PMC248798

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2173789-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Thermally inactivated simian virus 40 tsA58 mutant T antigen cannot initiate viral DNA replication in vitro.PMID 2173789Read ↗

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