Monoclonal antibody to the human insulin receptor, but not insulin, stimulates S6 kinase via human insulin receptors mutated at three major tyrosine autophosphorylation sites.

Abstract excerpt

Studies were carried out to examine the role of the major insulin receptor tyrosine autophosphorylation sites in stimulation of S6 kinase activity. For these studies, we employed HTC rat hepatoma cells transfected with and expressing human insulin receptors. In cells transfected with and expressing a large number of normal human insulin receptors (HTC-IR cells), the sensitivity of cells to insulin to stimulate S6...

Topics

• Animals
• Antibodies, Monoclonal
• Calcium-Calmodulin-Dependent Protein Kinases
• Enzyme Activation
• Humans
• Insulin
• Kinetics
• Mutation
• Phosphorylation
• Protein Kinases
• Protein Serine-Threonine Kinases