Article
Normal insulin receptor substrate-1 phosphorylation in autophosphorylation-defective truncated insulin receptor. Evidence that phosphorylation of substrates might be sufficient for certain biological effects evoked by insulin.
The Journal of biological chemistry - 5 Aug 1993
Yamamoto-Honda R, Kadowaki T, Momomura K, Tobe K, Tamori Y, Shibasaki Y, Mori Y, Kaburagi Y, Koshio O, Akanuma Y
Abstract excerpt
A mutant human insulin receptor that lacked the 82 amino acids of the COOH terminus of the beta-subunit (del82) was studied. Both the wild type insulin receptor (HIR) and the mutant receptor were expressed in Chinese hamster ovary (CHO) cells by stable transfection. Autophosphorylation and tyrosine kinase activities toward exogenous substrates of solubilized and partially purified del82 were severely impaired....
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