Article
Normal insulin receptor substrate-1 phosphorylation in autophosphorylation-defective truncated insulin receptor. Evidence that phosphorylation of substrates might be sufficient for certain biological effects evoked by insulin.
The Journal of biological chemistry - 5 Aug 1993
Yamamoto-Honda R, Kadowaki T, Momomura K, Tobe K, Tamori Y, Shibasaki Y, Mori Y, Kaburagi Y, Koshio O, Akanuma Y
Abstract excerpt
A mutant human insulin receptor that lacked the 82 amino acids of the COOH terminus of the beta-subunit (del82) was studied. Both the wild type insulin receptor (HIR) and the mutant receptor were expressed in Chinese hamster ovary (CHO) cells by stable transfection. Autophosphorylation and tyrosi...
Topics
- Animals
- CHO Cells
- Cell-Free System
- Cricetinae
- Deoxyglucose
- Humans
- Insulin
- Kinetics
- Lectins
- Mutation
- Phosphorylation
- Precipitin Tests
- Receptor, Insulin
- Solubility