Article
Using proteinase trapping to detect revertants of inactive rhinoviral 2A proteinase mutants.
Biological chemistry Hoppe-Seyler - 1 Jul 1992
Luderer M, Liebig H D, Sommergruber W, Blaas D, Kuechler E, Skern T
Abstract excerpt
The 2A proteinase of human rhinovirus 2 cleaves itself off the growing polyprotein at its own N terminus during translation; this property was used to develop an in vivo screening system with the lacZ gene fragment of M13mp18. The fusion of an active 2A proteinase to the C-terminus of the alpha-fragment did not affect alpha-complementation, as the proteinase cleaved itself off the alpha-fragment. However, an...
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