Using proteinase trapping to detect revertants of inactive rhinoviral 2A proteinase mutants.

doi:10.1515/bchm3.1992.373.2.523

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Article

Using proteinase trapping to detect revertants of inactive rhinoviral 2A proteinase mutants.

Biological chemistry Hoppe-Seyler - 1 Jul 1992

Luderer M, Liebig H D, Sommergruber W, Blaas D, Kuechler E, Skern T

Abstract excerpt

The 2A proteinase of human rhinovirus 2 cleaves itself off the growing polyprotein at its own N terminus during translation; this property was used to develop an in vivo screening system with the lacZ gene fragment of M13mp18. The fusion of an active 2A proteinase to the C-terminus of the alpha-f...

Topics

Bacteriophages
Cysteine Endopeptidases
DNA, Bacterial
Escherichia coli
Gene Amplification
Genetic Complementation Test
Mutagenesis
Mutation
Polymerase Chain Reaction
Rhinovirus
Viral Fusion Proteins
Viral Proteins

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