Article
Proteinase trapping: screening for viral proteinase mutants by alpha complementation.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jul 1991
Liebig H D, Skern T, Luderer M, Sommergruber W, Blaas D, Kuechler E
Abstract excerpt
Many virally encoded proteinases cleave themselves out of a polyprotein, with cleavage occurring usually at their own N terminus. This property was used to develop an in vivo screening system using the lacZ gene fragment of M13mp18. When a fusion protein of the alpha fragment of beta-galactosidase and an active 2A proteinase of human rhinovirus 2 was expressed, alpha complementation was not affected, as the 2A...
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