Article
Proteinase trapping: screening for viral proteinase mutants by alpha complementation.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jul 1991
Liebig H D, Skern T, Luderer M, Sommergruber W, Blaas D, Kuechler E
Abstract excerpt
Many virally encoded proteinases cleave themselves out of a polyprotein, with cleavage occurring usually at their own N terminus. This property was used to develop an in vivo screening system using the lacZ gene fragment of M13mp18. When a fusion protein of the alpha fragment of beta-galactosidas...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Blotting, Western
- Chromosome Deletion
- Endopeptidases
- Escherichia coli
- Genetic Complementation Test
- Genetic Vectors
- Mutagenesis, Site-Directed
- Mutation
- Polymerase Chain Reaction
- Recombinant Fusion Proteins
- Rhinovirus
- Transfection
- Viruses
- beta-Galactosidase