Generation and effective enrichment of selectable human cytomegalovirus mutants using site-directed insertion of the neo gene.

doi:10.1016/0378-1119(93)90416-z

Back to search

Article

Generation and effective enrichment of selectable human cytomegalovirus mutants using site-directed insertion of the neo gene.

Gene - 25 Aug 1993

Wolff D, Jahn G, Plachter B

Abstract excerpt

Studies on the biology and function of human cytomegalovirus (HCMV) genes have been hampered by the limited number of viral mutants available for genetic analyses. We have developed a simple procedure to generate and enrich for HCMV recombinants. By inserting the bacterial neo gene, encoding neom...

Topics

Blotting, Southern
Cells, Cultured
Cytomegalovirus
DNA, Viral
Genes, Bacterial
Humans
Kanamycin
Kanamycin Kinase
Mutagenesis, Site-Directed
Phenotype
Phosphorylation
Phosphotransferases
Recombination, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.