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Article

Generation and effective enrichment of selectable human cytomegalovirus mutants using site-directed insertion of the neo gene.

Gene - 25 Aug 1993

Wolff D, Jahn G, Plachter B

Abstract excerpt

Studies on the biology and function of human cytomegalovirus (HCMV) genes have been hampered by the limited number of viral mutants available for genetic analyses. We have developed a simple procedure to generate and enrich for HCMV recombinants. By inserting the bacterial neo gene, encoding neomycin/kanamycin phosphotransferase, into the large HCMV DNA genome using homologous recombination, selectable mutants of...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Mutagenesis, Site-Directed

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Source

Identifiers

PMID: 8395449
DOI: 10.1016/0378-1119(93)90416-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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