Article
Analysis of the functional significance of amino acid residues in the putative NTP-binding pattern of the poliovirus 2C protein.
The Journal of general virology - 1 Aug 1992
Teterina N L, Kean K M, Gorbalenya A E, Agol V I, Girard M
Abstract excerpt
The amino acid sequence of the poliovirus 2C protein contains two highly conserved stretches, GSPGTGKS136 and MDD177, which correspond to the consensus 'A' and 'B' motifs (GXXXXGKS/T and DD/E, respectively) found in nucleoside triphosphate-binding proteins. To assess the functional importance of these amino acid sequences, we changed conserved and non-conserved amino acids. The replacement of the non-conserved...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Carrier Proteins
- Cloning, Molecular
- Escherichia coli
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides