Three poliovirus 2B mutants exhibit noncomplementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus.

Abstract excerpt

Many functions of the poliovirus genome in virally infected cells have been elucidated. However, the role of 2B (and of its precursor polypeptide, 2BC), encoded by the P2 region in the poliovirus genome, remains unknown. We have employed a genetic approach to examine the role of 2B in poliovirus-infected cells. We report here the phenotype of one previously isolated mutant in the 2B coding region, 2B201. In...

Topics

• Animals
• DNA, Viral
• Genetic Complementation Test
• Genotype
• HeLa Cells
• Humans
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Phenotype
• Poliovirus
• Protein Biosynthesis