Article
Transformation of rodent fibroblasts by herpes simplex virus: presence of morphological transforming region 1 (MTR 1) is not required for the maintenance of the transformed state.
International journal of cancer - 9 Jul 1992
Bauer G, Kahl S, Sawhney I S, Höfler P, Gerspach R, Matz B
Abstract excerpt
Studies on the mechanisms of transformation of mammalian cells by herpes simplex virus (HSV) in vitro have been prevented so far by the extremely low transformation frequencies obtained in monolayer culture. Here we present a transformation system that relies on the direct seeding in soft agar of infected single cells, thus avoiding negative interactions between normal and transformed cells. We took advantage of...
Topics
- Animals
- Cell Line, Transformed
- Cell Transformation, Neoplastic
- Cell Transformation, Viral
- DNA, Viral
- Fibroblasts
- Gene Expression
- Mice
- Mice, Inbred C3H
- Mutation