Article
Cloning of a protein binding to the most proximal Pit-1 binding element of prolactin gene from human pituitary cDNA library.
Molecular and cellular endocrinology - 30 Sept 2003
Fumoto Mariko, Okimura Yasuhiko, Sakagami Yoshio, Iguchi Genzo, Kishimoto Masahiko, Takahashi Yutaka, Kaji Hidesuke, Chihara Kazuo
Abstract excerpt
A human pituitary cDNA library was screened using a yeast one-hybrid system to find a factor binding Pit-1 binding elements in the PRL gene other than Pit-1. Beside colonies containing Pit-1 or Oct-1 cDNA, three colonies contained mPOU cDNA, a member of the POU protein family. Immunohistochemical analysis showed mPOU-like immunoreactivity was present in human PRL-producing pituitary tumors but not in...
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