Article
[HLA-A genotyping by oligonucleotide arrays].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Jul 2003
Hu Shou-wang, Zhang Fan, Wang Hui, Geng Yong-yao, Wang Sheng-qi
Abstract excerpt
OBJECTIVE: To investigate HLA genotyping by oligonucleotide arrays. METHOD: Unsymmetrical PCR was used to amplify HLA-A gene exon 2, 3. The PCR products were used as templates for hybridization. The oligonucleotide probes were spotted on glass to make microarrays. High signal and specific probes were selected. The effects of the length and location of probes on hybridization signal were studied. A set of computer...
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