Article
[Investigation of genotyping HLA DRB1 gene using oligoneucleotide arrays].
Yi chuan xue bao = Acta genetica Sinica - 1 Jan 2001
Chen J N, Li Y, Li Y L, Qin H Y, Li R Y, Cao H M, Xie Y, Mao Y M
Abstract excerpt
We have developed a method performed on an oligoneucleotide array for genotyping HLA DRB1. The routine method Phenol-chloroform was used to extract genome DNA of standard samples. A pair of sense and antisense primers were designed according to the sequence of DRB1 exon2, then the primers and the Cy5-dCTP were used in the following PCR, thus the PCR products were labelled with Cy5. Many genotyping probes which...
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