Article
[Development of oligoneucleotide arrays for HLA-DR53-associated group genotyping].
Zhonghua wai ke za zhi [Chinese journal of surgery] - 1 Mar 2003
Xiao Jia-quan, Li Cheng-tao, Tan Jian-ming, Kang Min-hua, Li Yao
Abstract excerpt
OBJECTIVE: To develop a method performed on an oligonucleotide array for HLA-DR53 group genotyping. METHODS: According to the specific allelic frequency and sequence of HLA-DRB loci in Chinese Han population, HLA-DR53 group typing probes which were immobilized on a glass supports were synthesized. A pair of group-special primers labeled by the Cy5-dCTP were designed, and the primers were used in the PCR, thus the...
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