Article
Oligonucleotide microarray for HLA-DRB1 genotyping: preparation and clinical evaluation.
Tissue antigens - 1 May 2005
Zhang F, Hu S W, Huang J, Wang H, Zhang W, Geng Y Y, Wang S Q
Abstract excerpt
In this study, HLA-DRB1 gene was genotyped by using the microarray technique. Oligonucleotide probes were designed based on partial sequences of various genotypes of HLA-DRB1, and were fixed on a silylated slide to form a microarray. The second exon of HLA-DRB1 gene in the extracted genomic DNA samples was amplified and labelled by means of polymerase chain reaction (PCR); then it was hybridized to the...
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