Article
[HLA-DQA1 genotyping by using oligonucleotide microarrays].
Zhongguo shi yan xue ye xue za zhi - 1 Feb 2006
Wang Tong, Wang Tian-Jiao, He Qun, Zhang Yu-Kui, Ma Jia-Ming, Hou Wei-Jian, Wang Shao-Cheng, Pan Zhong-Cheng, Zhao Yu-Jie
Abstract excerpt
In order to fabricate the HLA-DQA1 genotyping chip and develop an integrated, parallel technical platform to type HLA system, a pair of primers and a set of probes were designed according to the sequences of HLA-DQA1 exon 2, where the polymorphism is concentrated. The oligonucleotide chip was made with the methods developed in our laboratory. The target DNA was asymmetrically amplified with the labeled sense...
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