Article
Monitoring manufacturing process yields, purity and stability of structural variants of PEGylated staphylokinase mutant SY161 by quantitative reverse-phase chromatography.
Biomedical chromatography : BMC - 1 Jul 2003
Johnson Catharine, Royal Mabel, Moreadith Randall, Bedu-Addo Frank, Advant Siddharth, Wan Min, Conn Greg
Abstract excerpt
Staphylokinase variant SY161 is a recombinant mutant of the Staphylococcus aureus polypeptide staphylokinase (Sak), and is currently in human clinical trials as a thrombolytic agent. The 15 kDa single chain SY161 protein is expressed as a soluble cytoplasmic product in E. coli with a single cysteine inserted near the N-terminus. The protein as extracted from E. coli is a mixture of both monomeric and...
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