Article
Engineering and production of streptokinase in a Bacillus subtilis expression-secretion system.
Applied and environmental microbiology - 1 Feb 1994
Wong S L, Ye R, Nathoo S
Abstract excerpt
Streptokinase is one of the major blood-clot-dissolving agents used in many medical treatments. With the cloned streptokinase gene (skc) available, production of the secreted streptokinase from various Bacillus subtilis strains was studied. The use of the six-extracellular-protease-deficient strain, WB600, greatly improved the production yield of the secreted streptokinase. A modified skc which has the original...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Culture Media
- Drug Resistance
- Endopeptidases
- Genetic Engineering
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Plasmids