Article
Refolding of a staphylokinase variant y1-Sak by reverse dilution.
Applied biochemistry and biotechnology - 1 Oct 2008
He Jintian, Wang Gaizhen, Xu Ruiguang, Feng Jinlin, Wang Jinlong, Su Huabo, Song Houyan
Abstract excerpt
To develop more potent thrombolytic agents with fibrinolytic and antiplatelet aggregation activity, staphylokinase (Sak) variant Y1-Sak, a recombinant mutant of the Staphylococcus aureus protein Sak, was constructed. Y1-Sak formed an insoluble inclusion body when overexpressed in Escherichia coli strain JF1125. To obtain an optimized refolding process, dilution refolding was used to optimize refolding conditions....
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