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Article

The Impact of Cysteine Substitutions on TGF-β3 Expression, Purification, Folding, and Activity.

International journal of molecular sciences - 6 Mar 2026

Albawaana Amal, Day Anil, Lu Hui

Abstract excerpt

Transforming growth factor beta 3 (TGF-β3) is a homodimeric cytokine with potential therapeutic applications in wound healing, tissue engineering and regenerative medicine. Production of recombinant TGF-β3 in Escherichia coli faces significant challenges due to TGF-β3's propensity for misfolding and aggregation, driven by a high disulfide bond content and low aqueous solubility. To address these limitations, the...

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Amino Acid Substitution
Cysteine
Disulfides
Escherichia coli
Protein Folding

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Multimerization

Recombinant Proteins

Transforming Growth Factor beta3

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Open in PubMed Open full text

Identifiers

PMID: 41828639
DOI: 10.3390/ijms27052422
PMCID: PMC12986189

Genes

C7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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