Article
Proteosynthetic activity of immobilized Staphylococcus aureus V8 protease: application in the semisynthesis of molecular variants of alpha-globin.
Analytical biochemistry - 2 Mar 1991
Sahni G, Mallia A K, Acharya A S
Abstract excerpt
The proteosynthetic activity of Staphylococcus aureus V8 protease (endoproteinase Glu-C) immobilized onto cross-linked agarose beads by reductive alkylation procedure has been investigated. The overall substrate specificity of the enzyme, as judged by peptide mapping of performic acid oxidized RN...
Topics
- Chromatography, High Pressure Liquid
- Enzymes, Immobilized
- Genetic Variation
- Globins
- Heme
- Humans
- Peptide Fragments
- Serine Endopeptidases
- Substrate Specificity
