Article
Comparison of two PCR-based methods and automated DNA sequencing for prop-1 genotyping in Ames dwarf mice.
Mutation research - 25 Jul 2003
Gerstner Arpad, DeFord James H, Papaconstantinou John
Abstract excerpt
Ames dwarfism is caused by a homozygous single nucleotide mutation in the pituitary specific prop-1 gene, resulting in combined pituitary hormone deficiency, reduced growth and extended lifespan. Thus, these mice serve as an important model system for endocrinological, aging and longevity studies. Because the phenotype of wild type and heterozygous mice is undistinguishable, it is imperative for successful...
Topics
- Animals
- Automation
- Genotype
- Homeodomain Proteins
- Mice
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA