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Article

Development of an efficient genotyping method to detect obese mutation in the mouse leptin gene for use in SPF barrier facilities.

The Journal of veterinary medical science - 1 Jan 2013

Ayabe Hiroaki, Ikeda Shogo, Maruyama Shigeri, Shioyama Seiji, Kikuchi Mayumi, Kawaguchi Ayako, Yamada Tetsuo, Ikeda Takuya

Abstract excerpt

We have developed a rapid and efficient genotyping method for detection of the mouse leptin obese mutation (Lep(ob)) using tetra-primer amplification refractory mutation system-polymerase chain reaction (tetra-primer ARMS-PCR). In this method, whole blood collected onto gamma-ray sterilized Flinders Technology Associates (FTA) filter paper is used as PCR template without a DNA purification step. Three genotypes...

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Identifiers

PMID: 23229540
DOI: 10.1292/jvms.12-0348

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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