Article
A new method of genotyping MDX4CV mice by PCR-RFLP analysis.
Muscle & nerve - 1 Sept 2017
Tichy Elisia D, Mourkioti Foteini
Abstract excerpt
INTRODUCTION: The mdx4cv mouse is a common model to study Duchenne muscular dystrophy. The most used methodology to identify the genotype of these mice is Sanger DNA sequencing. METHODS: Here, we provide a simple, cost-effective alternative approach to identify the wild-type, heterozygous, or homozygous/hemizygous genotypes of these mice, using commonly available laboratory equipment and reagents. RESULTS: Our...
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