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Rapid and simple screening of transgenic mice: novel extraction-free, filter-based PCR genotyping from blood samples.

Acta biochimica Polonica - 1 Jan 2006

Suematsu Naoya, Isohashi Fumihide

Abstract excerpt

We evaluated the effectiveness of using Flinders Technology Associates (FTA) filter paper for the polymerase chain reaction (PCR) genotyping of transgenic mice. Tail prick blood sample dried on an FTA filter disc was processed for genomic PCR. It is easy and rapid to prepare DNA templates because the protocol is extraction-free and only requires minimal handling of wash briefly bloodstained FTA filter discs....

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PMID: 16951742

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