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A rapid and inexpensive genotyping method using dried blood spots for mutational analysis in a mutant mouse model: an update.

Molecular biology reports - 1 Sept 2022

Romano Antonella, Zuchegna Candida, Zannini Giuseppa, Messina Samantha, Fusco Clorinda, Pezone Antonio, Grillo Roberta, de Candia Paola, Matarese Giuseppe, Porcellini Antonio

Abstract excerpt

BACKGROUND: Dried blood spot (DBS) testing is a well-known method of bio-sampling by which blood samples are blotted and dried on filter paper. The dried samples can then be analyzed by several techniques such as DNA amplification and HPLC. We have developed a non-invasive sampling followed by an alternative protocol for genomic DNA extraction from a drop of blood adsorbed on paper support. This protocol consists...

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PMID: 35733059
DOI: 10.1007/s11033-022-07649-x

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