Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Constitutive precoupling to G(i) and increased agonist potency in the alpha(2B)-adrenoceptor.

Biochemical and biophysical research communications - 11 Jul 2003

Ge Huifang, Scheinin Mika, Kallio Jaana

Abstract excerpt

The human alpha(2B)-adrenoceptor (alpha(2B)-AR) was mutated by substituting the D(3.49) aspartate in position 109 with an alanine (alpha(2B)-D109A) in the conserved DRY sequence at the cytoplasmic face of TM3. We studied the effects of the mutation on agonist binding and on receptor activation in CHO cells, including possible inverse agonism monitored by measuring intracellular Ca(2+) concentrations...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Motifs
Share to Aspartic Acid
Share to CHO Cells

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cytoplasm

Share to GTP-Binding Protein alpha Subunits, Gi-Go

Share to Ligands

Share to Pertussis Toxin

Share to Receptors, Adrenergic, alpha-2

Source

Identifiers

PMID: 12821136
DOI: 10.1016/s0006-291x(03)01094-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Constitutive precoupling to G(i) and increased agonist potency in the alpha(2B)-adrenoceptor.PMID 12821136Read ↗

Constitutive activity of G-protein-coupled receptors: cause of disease and common property of wild-type receptors.Semantic proximity Read ↗

Inverse agonist activity at the alpha(2A)-adrenergic receptor.Semantic proximity Read ↗

Mutagenesis and peptide analysis of the DRY motif in the alpha2A adrenergic receptor: evidence for alternate mechanisms in G protein-coupled receptors.Semantic proximity Read ↗

Constitutive activity and inverse agonism at the alpha1adrenoceptors.Semantic proximity Read ↗

G-protein activation by putative antagonists at mutant Thr373Lys alpha2A adrenergic receptors.Semantic proximity Read ↗

Constitutive activity of receptors coupled to guanine nucleotide regulatory proteins.Semantic proximity Read ↗

An asn to lys polymorphism in the third intracellular loop of the human alpha 2A-adrenergic receptor imparts enhanced agonist-promoted Gi coupling.Semantic proximity Read ↗

Lessons from constitutively active mutants of G protein-coupled receptors.Semantic proximity Read ↗

Coupling of a mutated form of the human beta 2-adrenergic receptor to Gi and Gs. Requirement for multiple cytoplasmic domains in the coupling process.Semantic proximity Read ↗

Constitutively active mutants of the alpha 2-adrenergic receptor.Semantic proximity Read ↗

Characteristics for a salt-bridge switch mutation of the alpha(1b) adrenergic receptor. Altered pharmacology and rescue of constitutive activity.Semantic proximity Read ↗

A cannabinoid receptor 1 mutation proximal to the DRY motif results in constitutive activity and reveals intramolecular interactions involved in receptor activation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.