Article
Coupling of a mutated form of the human beta 2-adrenergic receptor to Gi and Gs. Requirement for multiple cytoplasmic domains in the coupling process.
The Journal of biological chemistry - 15 Mar 1991
Liggett S B, Caron M G, Lefkowitz R J, Hnatowich M
Abstract excerpt
We constructed five genes encoding mutant human beta 2-adrenergic receptor sequence (beta 2AR) which contained 12-22 amino acid substitutions with corresponding sequence from the human alpha 2AAR in order to assess the receptor domains involved in Gs versus Gi recognition and coupling. Mutant bet...
Topics
- Adenosine Diphosphate Ribose
- Adenylyl Cyclases
- Amino Acid Sequence
- Binding, Competitive
- Cytoplasm
- GTP-Binding Proteins
- Humans
- Molecular Sequence Data
- Mutation
- Pertussis Toxin
- Radioligand Assay
- Receptors, Adrenergic, beta
- Virulence Factors, Bordetella