Article
Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast.
Molecular and cellular biology - 1 Jun 2003
McPheeters David S, Muhlenkamp Peggy
Abstract excerpt
A series of efficiently spliced pre-mRNA substrates containing single 4-thiouridine residues were used to monitor RNA-protein interactions involving the branch site-3' splice site-3' exon region during yeast pre-mRNA splicing through cross-linking analysis. Prior to the assembly of the prespliceosome, Mud2p and the branch point bridging protein cross-link to a portion of this region in an ATP-independent fashion....
Topics
- Adenosine Triphosphatases
- Catalysis
- Cross-Linking Reagents
- DEAD-box RNA Helicases
- Exons
- Fungal Proteins
- Introns
- Ligands
- Models, Genetic
- Mutation
- Protein Binding