High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris.

doi:10.3390/ijms21010279

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Article

High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris.

International journal of molecular sciences - 31 Dec 2019

Zhou Qinghua, Su Zhixin, Jiao Liangcheng, Wang Yao, Yang Kaixin, Li Wenjuan, Yan Yunjun

Abstract excerpt

As a promising biocatalyst, Yarrowia lipolytica lipase 2 (YlLip2) is limited in its industrial applications due to its low thermostability. In this study, a thermostable YlLip2 mutant was overexpressed in Pichia pastoris and its half-life time was over 30 min at 80 °C. To obtain a higher protein...

Topics

Enzyme Stability
Fungal Proteins
Hot Temperature
Lipase
Mutation
Pichia
Recombinant Proteins
Yarrowia

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