Article
High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris.
International journal of molecular sciences - 31 Dec 2019
Zhou Qinghua, Su Zhixin, Jiao Liangcheng, Wang Yao, Yang Kaixin, Li Wenjuan, Yan Yunjun
Abstract excerpt
As a promising biocatalyst, Yarrowia lipolytica lipase 2 (YlLip2) is limited in its industrial applications due to its low thermostability. In this study, a thermostable YlLip2 mutant was overexpressed in Pichia pastoris and its half-life time was over 30 min at 80 °C. To obtain a higher protein secretion level, the gene dosage of the mutated lip2 gene was optimized and the lipase activity was improved by about...
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