Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris.

Gene - 29 Apr 1997

Sreekrishna K, Brankamp R G, Kropp K E, Blankenship D T, Tsay J T, Smith P L, Wierschke J D, Subramaniam A, Birkenberger L A

Abstract excerpt

Numerous heterologous proteins have been produced at greater than gram per liter levels in the methylotrophic yeast, Pichia pastoris, using the methanol oxidase promoter. The factors that drastically influence protein production in this system include: copy number of the expression cassette, site and mode of chromosomal integration of the expression cassette, mRNA 5'- and 3'-untranslated regions (UTR),...

Topics

Cloning, Molecular
Gene Dosage
Glycoproteins
Humans
Metalloendopeptidases
Methanol
Phenotype
Pichia
Protein Biosynthesis
RNA, Messenger
Recombinant Fusion Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tissue Inhibitor of Metalloproteinases

Source

Identifiers

PMID: 9185849
DOI: 10.1016/s0378-1119(96)00672-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-9185849-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris.PMID 9185849Read ↗

Recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.Semantic proximity Read ↗

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway.Semantic proximity Read ↗

Pichia pastoris Mut(S) strains are prone to misincorporation of O-methyl-L-homoserine at methionine residues when methanol is used as the sole carbon source.Semantic proximity Read ↗

Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris.Semantic proximity Read ↗

The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures.Semantic proximity Read ↗

Evaluation of Mut(S) and Mut⁺ Pichia pastoris strains for membrane-bound catechol-O-methyltransferase biosynthesis.Semantic proximity Read ↗

Structural characterization of the α-mating factor prepro-peptide for secretion of recombinant proteins in Pichia pastoris.Semantic proximity Read ↗

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement.Semantic proximity Read ↗

Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris.Semantic proximity Read ↗

Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.