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Article

A high-throughput gene disruption methodology for the entomopathogenic fungus Metarhizium robertsii.

PloS one - 1 Jan 2014

Xu Chuan, Zhang Xing, Qian Ying, Chen Xiaoxuan, Liu Ran, Zeng Guohong, Zhao Hong, Fang Weiguo

Abstract excerpt

Systematic gene disruption is a direct way to interrogate a fungal genome to functionally characterize the full suite of genes involved in various biological processes. Metarhizium robertsii is extraordinarily versatile, and it is a pathogen of arthropods, a saprophyte and a beneficial colonizer of rhizospheres. Thus, M. robertsii can be used as a representative to simultaneously study several major lifestyles...

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PMID: 25222118
DOI: 10.1371/journal.pone.0107657
PMCID: PMC4164657

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