Article
A system for deletion and complementation of Candida glabrata genes amenable to high-throughput application.
Gene - 12 Jun 2002
Willins Debra Aker, Shimer George H, Cottarel Guillaume
Abstract excerpt
We describe a method for deleting or modifying genes from the pathogenic fungus Candida glabrata as well as a companion vector for complementation or ectopic expression experiments. A linear deletion fragment generated by polymerase chain reaction was used to replace a gene of interest with the C. glabrata gene encoding imidazoleglycerol-phosphate dehydratase (HIS3). As test cases, the chromosomal loci of the C....
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