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Article

Mini-blaster-mediated targeted gene disruption and marker complementation in Candida albicans.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Ganguly Shantanu, Mitchell Aaron P

Abstract excerpt

Several gene disruption strategies have been described in Candida albicans to create homozygous mutants. We describe here a recyclable mini-blaster cassette containing C. albicans URA3 gene and 200-bp flanking repeats that is useful for disruption of C. albicans genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the HIS1 gene locus. This strategy of creating gene...

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Identifiers

PMID: 22328365
DOI: 10.1007/978-1-61779-539-8_2
PMCID: PMC3918946

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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