Article
p19Arf-p53 tumor suppressor pathway regulates cell motility by suppression of phosphoinositide 3-kinase and Rac1 GTPase activities.
The Journal of biological chemistry - 18 Apr 2003
Guo Fukun, Gao Yuan, Wang Lei, Zheng Yi
Abstract excerpt
The p19(Arf)-p53 tumor suppressor pathway plays a critical role in cell-cycle checkpoint control and apoptosis, whereas Rho family small GTPases are key regulators of actin structure and cell motility. By using primary mouse embryonic fibroblasts that lack Arf, p53, or both, we studied the involvement of the p19(Arf)-p53 pathway in the regulation of cell motility and its relationship with Rho GTPases. Deletion of...
Topics
- Animals
- Blotting, Western
- Cell Movement
- Cells, Cultured
- Culture Media, Serum-Free
- Cyclin-Dependent Kinase Inhibitor p16
- Enzyme Inhibitors
- Fibroblasts
- Gene Deletion
- Green Fluorescent Proteins