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Article

Structure-Based Improvement of the Biophysical Properties of Immunoglobulin V<sub>H</sub> Domains with a Generalizable Approach

25 Jan 2003

Abstract excerpt

In a systematic study of V gene families carried out with consensus V(H) and V(L) domains alone and in combinations in the scFv format, we found comparatively low expression yields and lower cooperativity in equilibrium unfolding in antibody fragments containing V(H) domains of human germline families 2, 4, and 6. From an analysis of the packing of the hydrophobic core, the completeness of charge clusters, the...

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PMID: 12578364
DOI: 10.1021/bi026448p

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