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On the humanization of VHHs: Prospective case studies, experimental and computational characterization of structural determinants for functionality

18 Oct 2024

Abstract excerpt

The humanization of camelid-derived variable domain heavy chain antibodies (VHHs) poses challenges including immunogenicity, stability, and potential reduction of affinity. Critical to this process are complementarity-determining regions (CDRs), Vernier and Hallmark residues, shaping the three-dimensional fold and influencing VHH structure and function. Additionally, the presence of non-canonical disulfide bonds...

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PMID: 39422475
DOI: 10.1002/pro.5176

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