Article
A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris.
Applied and environmental microbiology - 1 Feb 2003
Hanquier José, Sorlet Yannick, Desplancq Dominique, Baroche Laurence, Ebtinger Marc, Lefèvre Jean-François, Pattus Franc, Hershberger Charles L, Vertès Alain A
Abstract excerpt
We produced bovine trypsinogen in the yeast Pichia pastoris. Little or no trypsinogen was detected when the gene with its native leader sequence was expressed under the control of the strong aox1 promoter, suggesting that expression of the wild-type bovine trypsinogen was toxic to the cells. We a...
Topics
- Animals
- Binding Sites
- Cattle
- Culture Media
- Enzyme Activation
- Enzyme Precursors
- Mutation
- Pichia
- Recombinant Proteins
- Trypsin
- Trypsinogen