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Article

A rapid and effective procedure for screening protease mutants.

Protein engineering - 1 Jan 1996

Venekei I, Hedstrom L, Rutter W J

Abstract excerpt

We describe a simple and effective procedure to screen for active proteases among a large number of mutants. First, the mutants are genetically tested by the protease activity produced in the periplasm of transformed bacteria which supplies the cells with a nitrogen source by hydrolyzing a protein applied to plates. Then a less sensitive activity staining and an X-ray film digestion assay are used to verify and...

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PMID: 9053907
DOI: 10.1093/protein/9.1.85

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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