A rapid and effective procedure for screening protease mutants.

doi:10.1093/protein/9.1.85

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Article

A rapid and effective procedure for screening protease mutants.

Protein engineering - 1 Jan 1996

Venekei I, Hedstrom L, Rutter W J

Abstract excerpt

We describe a simple and effective procedure to screen for active proteases among a large number of mutants. First, the mutants are genetically tested by the protease activity produced in the periplasm of transformed bacteria which supplies the cells with a nitrogen source by hydrolyzing a protein applied to plates. Then a less sensitive activity staining and an X-ray film digestion assay are used to verify and...

Topics

Amino Acid Sequence
Animals
Blotting, Western
Cell Division
Chymotrypsin
Electrophoresis, Polyacrylamide Gel
Endopeptidases
Escherichia coli
Gene Library
Kinetics
Molecular Sequence Data
Mutagenesis
Mutation
Protein Engineering
Rats
Recombinant Proteins
Sequence Analysis
Sequence Homology, Amino Acid

Source

Open in PubMed

Identifiers

PMID: 9053907
DOI: 10.1093/protein/9.1.85

Provenance

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