Development of mutant human immunoreactive trypsinogen 1 (IRT1) and mutant human immunoreactive trypsinogen 2 (IRT2) for use in immunoassays.

doi:10.1016/j.pep.2020.105572

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Article

Development of mutant human immunoreactive trypsinogen 1 (IRT1) and mutant human immunoreactive trypsinogen 2 (IRT2) for use in immunoassays.

Protein expression and purification - 1 May 2020

Divyapicigil Mustafa, Pirincci Gokturk Serife Seyda, Ergenoglu Bengu, Yucel Fatima, Akcael Esin Aslankaraoglu

Abstract excerpt

Immunoreactive Trypsinogen (IRT) is a protein-based pancreatic proenzyme that has an important role in protein digestion in humans. In human body, once IRT present in the small intestine, the proteolytic cleavage activates trypsinogen into trypsin. When IRT is in the active form, it is capable to cleave antibodies, other proteins and even itself while it is desired to use in immunoassays. According to the...

Topics

Antigens
Blotting, Western
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay
Mutation
Pichia
Protein Isoforms
Recombinant Proteins
Trypsin
Trypsinogen

Source

Open in PubMed

Identifiers

PMID: 31972264
DOI: 10.1016/j.pep.2020.105572

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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