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Article

A genetic screen to identify variants of bovine pancreatic trypsin inhibitor with altered folding energetics.

Proteins - 1 Jan 1990

Coplen L J, Frieden R W, Goldenberg D P

Abstract excerpt

A genetic screening procedure has been developed to identify mutant forms of bovine pancreatic trypsin inhibitor (BPTI) that can fold to an active conformation but are inactivated more rapidly than the wild-type protein. Small cultures of Escherichia coli containing plasmids with mutagenized BPTI genes were grown in microtiter plates, lysed, and treated with dithiothreitol (DTT). Under these conditions, unfolding...

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Identifiers

PMID: 1691852
DOI: 10.1002/prot.340070103

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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