A genetic screen to identify variants of bovine pancreatic trypsin inhibitor with altered folding energetics.

doi:10.1002/prot.340070103

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Article

A genetic screen to identify variants of bovine pancreatic trypsin inhibitor with altered folding energetics.

Proteins - 1 Jan 1990

Coplen L J, Frieden R W, Goldenberg D P

Abstract excerpt

A genetic screening procedure has been developed to identify mutant forms of bovine pancreatic trypsin inhibitor (BPTI) that can fold to an active conformation but are inactivated more rapidly than the wild-type protein. Small cultures of Escherichia coli containing plasmids with mutagenized BPTI...

Topics

Amino Acid Sequence
Animals
Aprotinin
Chick Embryo
Dithiothreitol
Drug Resistance, Microbial
Genetic Markers
Genetic Variation
Kinetics
Molecular Sequence Data
Mutation
Protein Conformation

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