Article
Evolution of trypsinogen activation peptides.
Molecular biology and evolution - 1 Nov 2003
Chen Jian-Min, Kukor Zoltán, Le Maréchal Cédric, Tóth Miklós, Tsakiris Laurent, Raguénès Odile, Férec Claude, Sahin-Tóth Miklós
Abstract excerpt
The activation peptide of mammalian trypsinogens contains a highly conserved tetra-aspartate sequence (D19-D20-D21-D22) preceding the K23-I24 scissile peptide bond, which is hydrolyzed as the first step in the activation process. Here, we examined the evolution and function of trypsinogen activation peptides through integrating functional characterization of disease-associated mutations with comparative genomic...
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Cations
- Enteropeptidase
- Enzyme Activation
- Evolution, Molecular
- Fishes
- Hydrolysis
- Lysine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oligopeptides
- Peptides
- Plasmids
- Recombinant Proteins
- Time Factors